Microbes get by with a little help from their friends
Related Articles
Weak ties and the value of social connections for autistic people as revealed during the COVID-19 pandemic
A diverse portfolio of social relationships matters for people’s wellbeing, including both strong, secure relationships with others (‘close ties’) and casual interactions with acquaintances and strangers (‘weak ties’). Almost all of autism research has focused on Autistic people’s close ties with friends, family and intimate partners, resulting in a radically constrained understanding of Autistic sociality. Here, we sought to understand the potential power of weak-tie interactions by drawing on 95 semi-structured interviews with Autistic young people and adults conducted during the COVID-19 pandemic. We analysed the qualitative data using reflexive thematic analysis within an essentialist framework. During the COVID-19 lockdowns, Autistic people deeply missed not only their close personal relationships but also their “incidental social contact” with acquaintances and strangers. These weak-tie interactions appear to serve similar functions for Autistic people as they do for non-autistic people, including promoting wellbeing. These findings have important implications both for future research into Autistic sociality and for the design of practical services and supports to enhance Autistic people’s opportunities to flourish.
Microbiome-based interventions to modulate gut ecology and the immune system
The gut microbiome lies at the intersection between the environment and the host, with the ability to modify host responses to disease-relevant exposures and stimuli. This is evident in how enteric microbes interact with the immune system, e.g., supporting immune maturation in early life, affecting drug efficacy via modulation of immune responses, or influencing development of immune cell populations and their mediators. Many factors modulate gut ecosystem dynamics during daily life and we are just beginning to realise the therapeutic and prophylactic potential of microbiome-based interventions. These approaches vary in application, goal, and mechanisms of action. Some modify the entire community, such as nutritional approaches or faecal microbiota transplantation, while others, such as phage therapy, probiotics, and prebiotics, target specific taxa or strains. In this review, we assessed the experimental evidence for microbiome-based interventions, with a particular focus on their clinical relevance, ecological effects, and modulation of the immune system.
Selection and enrichment of microbial species with an increased lignocellulolytic phenotype from a native soil microbiome by activity-based probing
Multi-omic analyses can provide information on the potential for activity within a microbial community but often lack specificity to link functions to cell, primarily offer potential for function or rely on annotated databases. Functional assays are necessary for understanding in situ microbial activity to better describe and improve microbiome biology. Targeting enzyme activity through activity-based protein profiling enhances the accuracy of functional studies. Here, we introduce a pipeline of coupling activity-based probing with fluorescence-activated cell sorting, culturing, and downstream activity assays to isolate and examine viable populations of cells expressing a function of interest. We applied our approach to a soil microbiome using two activity-based probes to enrich for communities with elevated activity for lignocellulose-degradation phenotypes as determined by four fluorogenic kinetic assays. Our approach efficiently separated and identified microbial members with heightened activity for glycosyl hydrolases, and by expanding this workflow to various probes for other function, this process can be applied to unique phenotype targets of interest.
Close relationship partners of impartial altruists do not report diminished relationship quality and are similarly altruistic
Impartial altruism is often considered a moral ideal but is rare in practice. Instead, generosity typically decreases as social distance increases, a phenomenon termed social discounting. Most people prefer this partiality in their close relationships and view impartial altruists as poorer relationship partners. This suggests real-world impartial altruism may be rare because it reduces—or is perceived to reduce—the quality of close relationships. To investigate this, we compared patterns of generosity and social relationship quality in a rare sample of individuals who had engaged in extraordinary real-world impartial altruism (altruistic kidney donors; n = 59) and their closest friend or family member (n = 59) to controls (n = 71) and their closest others (n = 71). We designed a direct test of third-party social discounting, which experimentally confirmed real-world altruists’ impartiality, finding that they are more likely than controls to split resources evenly between close and distant others rather than favoring close others. However, we found no statistically significant association between impartial altruism and social relationship quality. Instead, we found that altruists’ close others also show more impartiality than controls. This suggests value homophily (shared moral values) among altruists, which may represent a protective factor for close relationships in the context of impartial altruism.
From germ-free to wild: modulating microbiome complexity to understand mucosal immunology
The gut microbiota influences host responses at practically every level, and as research into host-microbe interactions expands, it is not surprising that we are uncovering similar roles for the microbiota at other barrier sites, such as the lung and skin. Using standard laboratory mice to assess host-microbe interactions, or even host intrinsic responses, can be challenging, as slight variations in the microbiota can affect experimental outcomes. When it comes to designing and selecting an appropriate level of microbial diversity and community structure for colonization of our laboratory rodents, we have more choices available to us than ever before. Here we will discuss the different approaches used to modulate microbial complexity that are available to study host-microbe interactions. We will describe how different models have been used to answer distinct biological questions, covering the entire microbial spectrum, from germ-free to wild.
Responses