Single-cell CRISPR screening in mouse brain
Category: CRISPR-Cas9 genome editing
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing
Robust expression of guide RNA (gRNA) is essential for successful implementation of CRISPR–Cas9 genome-editing methods. The gRNA components, such…
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) is a new approach for ex vivo genome editing of primary human cells. PERC …
Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing
RNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed b…