Generation of human fetal brain organoids and their CRISPR engineering for brain tumor modeling
The developing human brain displays unique features that are difficult to study in animal models. Current in vitro models based on human brain tiss…
Tag: CRISPR-Cas9 genome editing
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing
Robust expression of guide RNA (gRNA) is essential for successful implementation of CRISPR–Cas9 genome-editing methods. The gRNA components, such…
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells
Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) is a new approach for ex vivo genome editing of primary human cells. PERC …
Targeting BRCA1-deficient PARP inhibitor-resistant cells with nickases reveals nick resection as a cancer vulnerability
Tumors lacking the BRCA1 and BRCA2 (BRCA) hereditary breast cancer genes display heightened sensitivity to anti-cancer treatments, su…
Cellular dsRNA interactome captured by K1 antibody reveals the regulatory map of exogenous RNA sensing
RNA-binding proteins (RBPs) provide a critical post-transcriptional regulatory layer in determining RNA fate. Currently, UV crosslinking followed b…