Modulation of the human GlyT1 by clinical drugs and cholesterol
Glycine transporter 1 (GlyT1) is a key player in shaping extracellular glutamatergic signaling processes and holds promise for treating cognitive i…
Tag: Enzyme mechanisms
Aromatic side-chain crosslinking in RiPP biosynthesis
Peptide cyclization is a defining feature of many bioactive molecules, particularly in the ribosomally synthesized and post-translationally modifie…
The ISC machinery assembles [2Fe–2S] clusters by formation and fusion of [1Fe–1S] precursors
Iron–sulfur clusters are essential metallocofactors synthesized by multiprotein machineries via an unclear multistep process. Here we report a st…
Quinone extraction drives atmospheric carbon monoxide oxidation in bacteria
Diverse bacteria and archaea use atmospheric CO as an energy source for long-term survival. Bacteria use [MoCu]-CO dehydrogenases (Mo-CODH) to conv…
ADSL promotes autophagy and tumor growth through fumarate-mediated Beclin1 dimethylation
As an enzyme with a critical role in de novo purine synthesis, adenylosuccinate lyase (ADSL) expression is upregulated in various malignancies. How…
An efficient catalytic route in haem peroxygenases mediated by O2/small-molecule reductant pairs for sustainable applications
Haem peroxygenases are attractive biocatalysts for incorporating oxygen into organic molecules using H2O2. However, their pra…
Structure and mechanism of haem-dependent nitrogen–nitrogen bond formation in piperazate synthase
Molecules with nitrogen–nitrogen (N–N) bonds include diverse specialized metabolites from nature, but little is known about the underlying enzy…
Structure-function analysis of 2-sulfamoylacetic acid synthase in altemicidin biosynthesis
Altemicidin and its analogs are valuable sulfonamide antibiotics with valuable antitumor and antibacterial activities. Structures of altemicidin an…
Catalytic dwell oscillations complete the F1-ATPase mechanism
The F1-ATPase molecular motor rotates subunit-γ in 120° power strokes within its ring of three catalytic sites separated by catalytic …