CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d
Bacteriophages constitute one of the largest reservoirs of genes of unknown function in the biosphere. Even in well-characterized phages, the funct…
Tag: High-throughput screening
A genome-wide atlas of human cell morphology
A key challenge of the modern genomics era is developing empirical data-driven representations of gene function. Here we present the first unbiased…
Reproducible image-based profiling with Pycytominer
Advances in high-throughput microscopy have enabled the rapid acquisition of large numbers of high-content microscopy images. Next, whether by deep…
Active repression of cell fate plasticity by PROX1 safeguards hepatocyte identity and prevents liver tumorigenesis
Cell fate plasticity enables development, yet unlocked plasticity is a cancer hallmark. While transcription master regulators induce lineage-specif…
An enhanced framework for local genetic correlation analysis
Genetic correlation is a key parameter in the joint genetic model of complex traits, but it is usually estimated on a global genomic scale. Underst…
Systematic reconstruction of molecular pathway signatures using scalable single-cell perturbation screens
Recent advancements in functional genomics have provided an unprecedented ability to measure diverse molecular modalities, but predicting causal re…
Decoding heterogeneous single-cell perturbation responses
Understanding how cells respond differently to perturbation is crucial in cell biology, but existing methods often fail to accurately quantify and …