Standardized workflow for multiplexed charge detection mass spectrometry on orbitrap analyzers
Individual ion mass spectrometry (I2MS) is the Orbitrap-based extension of the niche mass spectrometry technique known as charge detecti…
Tag: Mass spectrometry
PEPPI-MS: gel-based sample pre-fractionation for deep top-down and middle-down proteomics
Top-down analysis of intact proteins and middle-down analysis of proteins subjected to limited digestion require efficient detection of traces of p…
An accessible workflow for high-sensitivity proteomics using parallel accumulation–serial fragmentation (PASEF)
Deep and accurate proteome analysis is crucial for understanding cellular processes and disease mechanisms; however, it is challenging to implement…
A multimodal imaging pipeline to decipher cell-specific metabolic functions and tissue microenvironment dynamics
Tissue microenvironments are extremely complex and heterogeneous. It is challenging to study metabolic interaction between the different cell types…
Plasma proteome variation and its genetic determinants in children and adolescents
Our current understanding of the determinants of plasma proteome variation during pediatric development remains incomplete. Here, we show that gene…
The ISC machinery assembles [2Fe–2S] clusters by formation and fusion of [1Fe–1S] precursors
Iron–sulfur clusters are essential metallocofactors synthesized by multiprotein machineries via an unclear multistep process. Here we report a st…
The measurement, regulation and biological activity of FAHFAs
Fatty acid esters of hydroxy fatty acids (FAHFAs) are bioactive lipids that are positively correlated with metabolic health in humans and mice. Sin…
High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads
In serology, each sample is typically tested individually, one antigen at a time. This is costly and time consuming. Serology techniques should ide…
ToF-SIMS sputter depth profiling of interphases and coatings on lithium metal surfaces
Lithium metal as a negative electrode material offers ten times the specific capacity of graphitic electrodes, but its rechargeable operation poses…