Detection of protein oligomers with nanopores
Powerful single-molecule approaches have been developed for the accurate measurement of protein oligomers, but they are often low throughput and li…
Tag: Single-molecule biophysics
Single-molecule protein sequencing with nanopores
Protein sequencing and the identification of post-translational modifications are key to understanding cellular signalling pathways and metabolic p…
Frequency locked whispering evanescent resonator (FLOWER) for biochemical sensing applications
Sensitive, rapid and label-free biochemical sensors are needed for many applications. In this protocol, we describe biochemical detection using FLO…
Circular Vectors as an efficient, fully synthetic, cell-free approach for preparing small circular DNA as a plasmid substitute for guide RNA expression in CRISPR–Cas9 genome editing
Robust expression of guide RNA (gRNA) is essential for successful implementation of CRISPR–Cas9 genome-editing methods. The gRNA components, such…
Oblique line scan illumination enables expansive, accurate and sensitive single-protein measurements in solution and in living cells
An ideal tool for the study of cellular biology would enable the measure of molecular activity nondestructively within living cells. Single-molecul…
Single-molecule live-cell RNA imaging with CRISPR–Csm
Understanding the diverse dynamic behaviors of individual RNA molecules in single cells requires visualizing them at high resolution in real time. …
METTL7A-mediated m6A modification of corin reverses bisphosphonates-impaired osteogenic differentiation of orofacial BMSCs
Bisphosphonate-related osteonecrosis of jaw (BRONJ) is characterized by impaired osteogenic differentiation of orofacial bone marrow stromal cells …
TCR catch bonds nonlinearly control CD8 cooperation to shape T cell specificity
Naturally evolved T-cell receptors (TCRs) exhibit remarkably high specificity in discriminating non-self antigens from self-antigens under dynamic …