TRIMming the nuclear pore
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Personalized bioceramic grafts for craniomaxillofacial bone regeneration
The reconstruction of craniomaxillofacial bone defects remains clinically challenging. To date, autogenous grafts are considered the gold standard but present critical drawbacks. These shortcomings have driven recent research on craniomaxillofacial bone reconstruction to focus on synthetic grafts with distinct materials and fabrication techniques. Among the various fabrication methods, additive manufacturing (AM) has shown significant clinical potential. AM technologies build three-dimensional (3D) objects with personalized geometry customizable from a computer-aided design. These layer-by-layer 3D biomaterial structures can support bone formation by guiding cell migration/proliferation, osteogenesis, and angiogenesis. Additionally, these structures can be engineered to degrade concomitantly with the new bone tissue formation, making them ideal as synthetic grafts. This review delves into the key advances of bioceramic grafts/scaffolds obtained by 3D printing for personalized craniomaxillofacial bone reconstruction. In this regard, clinically relevant topics such as ceramic-based biomaterials, graft/scaffold characteristics (macro/micro-features), material extrusion-based 3D printing, and the step-by-step workflow to engineer personalized bioceramic grafts are discussed. Importantly, in vitro models are highlighted in conjunction with a thorough examination of the signaling pathways reported when investigating these bioceramics and their effect on cellular response/behavior. Lastly, we summarize the clinical potential and translation opportunities of personalized bioceramics for craniomaxillofacial bone regeneration.
Vastly different energy landscapes of the membrane insertions of monomeric gasdermin D and A3
Gasdermin D and gasdermin A3 belong to the same family of pore-forming proteins and executors of pyroptosis, a form of programmed cell death. To unveil the process of their pore formation, we examine the energy landscapes upon insertion of the gasdermin D and A3 monomers into a lipid bilayer by extensive atomistic molecular dynamics simulations. We reveal a lower free energy barrier of membrane insertion for gasdermin D than for gasdermin A3 and a preference of gasdermin D for the membrane-inserted and of gasdermin A3 for the membrane-adsorbed state, suggesting that gasdermin D first inserts and then oligomerizes while gasdermin A3 oligomerizes and then inserts. Gasdermin D stabilizes itself in the membrane by forming more salt bridges and pulling phosphatidylethanolamine lipids and more water into the membrane. Gasdermin-lipid interactions support the pore formation. Our findings suggest that both the gasdermin species and the lipid composition modulate gasdermin pore formation.
The 11-month precursory fault activation of the 2019 ML 5.6 earthquake in the Weiyuan shale gas field, China
Anthropogenic activities such as hydraulic fracturing (HF) can trigger destructive earthquakes, the triggering mechanisms of which are still in debate. We utilize near-fault seismic recordings to study the preparatory phase of the 2019 ML 5.6 earthquake in the Weiyuan shale gas field (WSGF), Sichuan Basin, China, which struck 3 months after stimulation completion. This is one of the largest HF-triggered earthquakes worldwide. We observed an 11-month-long precursory fault activation, during which continuous seismicity illuminated the fault plane and provided warnings for a potential destructive earthquake. The fault activation is a consequence of injections in multiple HF well pads, with a variety of mechanisms at play. Numerical simulation reveals that the occurrence of the mainshock involves stress perturbation from post-injection aseismic slip. This work promotes our understanding of HF-induced earthquakes and suggests incorporating long-term near-fault observations and taking post-injection aseismic slip into account for effective hazard management.
Grover’s algorithm in a four-qubit silicon processor above the fault-tolerant threshold
Spin qubits in silicon are strong contenders for the realization of a practical quantum computer, having demonstrated single- and two-qubit gates with fidelities above the fault-tolerant threshold, and entanglement of three qubits. However, maintaining high-fidelity operations while increasing the qubit count remains challenging and therefore only two-qubit algorithms have been executed. Here we utilize a four-qubit silicon processor with all control fidelities above the fault-tolerant threshold and demonstrate a three-qubit Grover’s search algorithm with a ~95% probability of finding the marked state. Our processor is made of three phosphorus atoms precision-patterned into isotopically pure silicon, which localise one electron. The long coherence times of the qubits enable single-qubit fidelities above 99.9% for all qubits. Moreover, the efficient single-pulse multi-qubit operations enabled by the electron–nuclear hyperfine interaction facilitate controlled-Z gates between all pairs of nuclear spins with fidelities above 99% when using the electron as an ancilla. These control fidelities, combined with high-fidelity non-demolition readout of all nuclear spins, allow the creation of a three-qubit Greenberger–Horne–Zeilinger state with 96.2% fidelity. Looking ahead, coupling neighbouring nuclear spin registers, as the one shown here, via electron–electron exchange may enable larger, fault-tolerant quantum processors.
Fault–fracture mesh development produces tectonic tremor in fluid-overpressured serpentinized mantle wedge
Deep tectonic tremor occurs repeatedly at the base of a forearc mantle wedge corner, where a highly fluid-pressurized serpentinite shear zone is thought to develop. However, the deformation mechanisms that accommodate these tremors within the shear zone remain unclear. Here, we present observations of deformation experiments on water-saturated serpentinite conducted at pressure–temperature conditions relevant to the tremor zone. We find that increasing pore fluid pressure gradually decreases sample strength and leads to a transition in the deformation mechanism from frictional sliding on several fault surfaces to distributed extensional and extensional–shear fracturing. Combined with field observations of a shallow mantle-wedge-derived serpentinite shear zone, our experimental results suggest that numerous brittle failures developing simultaneously throughout the shear zone generate bursts of tectonic tremor. Furthermore, the recurrence interval of the tremors is likely controlled by the time required for the fractures to be hydrothermally sealed through serpentine precipitation.
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